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顶生系统/生物素化人2B4/CD244/SLAMF4蛋白，Avitag™,His标签（推荐用于生物淘洗）/25ug/2B4
- SynonymCD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
- SourceBiotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (2B4-H82E9) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # Q9BZW8-2).Predicted N-terminus: Cys 22Request for sequence
- Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 25.4 kDa. The protein migrates as 40-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.3-5 ng/mL (QC tested).
Immobilized Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) with a linear range of 0.3-5 ng/mL (Routinely tested).
- BackgroundNatural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
- References
- (1)Nakajima H., et al., 1999, Eur. J. Immunol. 29:1676-1683.
- (2)Kubin M.Z., et al., 1999, Eur. J. Immunol. 29:3466-3477.
- (3)Boles K.S., et al., 1999, Tissue Antigens 54:27-34.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
