- SynonymVTN,V75,VN,Vitronectin,S-protein,Serum-spreading factor
- SourceHuman Vitronectin, Tag Free (VIN-H5119) is expressed from E.coli cells. It contains AA Asp 20 -Arg 398 (Accession # P04004-1).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 43.2 kDa. The protein migrates as 48-50 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris-HCl, containing sucrose and Arginine, pH8.0 .
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Vitronectin, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Vitronectin, Tag Free (Cat. No. VIN-H5119) at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) with a linear range of 0.078-1.25 μg/mL (QC tested).
- BackgroundVitronectin is also known as S-protein, VN, VTN, V75. Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes. Blocking of Hic(a member of the pneumococcal surface protein C (PspC) family) by specific antiserum or genetic deletion significantly reduced pneumococcal binding to soluble and immobilised vitronectin and to Factor H, respectively. In addition, Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
- References
- (1)Kohler S, et al. 2015 Thromb Haemost. 113(1):125-42.
- (2)Salazar-Peláez LM, et al. 2015. PLoS One. 10(3):e0119717.
- (3)Paulsson M, et al. 2015. J Cyst Fibros. 14(5):600-7.
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