- SynonymUBE2H,UbcH2
- SourceHuman UbcH2, GST Tag (UBH-H513x) is expressed from E.coli cells. It contains AA Met 1 - Leu 183 (Accession # AAH06277).Predicted N-terminus: Met 1Request for sequence
- Molecular Characterization
This protein carries a GST tag at the N-terminus.
The protein has a calculated MW of 47.6 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human UbcH2, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundUbiquitin-conjugating enzyme E2 H (UBE2H), a member of the ubiquitin-conjugating enzyme family, is also known as ubiquitin carrier protein H, ubiquitin-conjugating enzyme E2-20K and ubiquitin-protein ligase H. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. At the same time, ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Furthermore,UBE2H can accept ubiquitin from the E1 complex and catalyze its covalent attachment to other proteins. In vitro,UBE2H can aslo catalyze "Lys-11"- and "Lys-48"-linked polyubiquitination, and it is capable, in vitro, to ubiquitinate histone H2A.
- References
- (1)Kaiser P., et al., 1994, J. Biol. Chem. 269:8797-8802.
- (2)David Y., et al., 2010, J. Biol. Chem. 285:8595-8604.
- (3)Wefes I et al., 1995, Proc Natl Acad Sci U S A. 92(11):4982-6.
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