- SynonymTSLP
- SourceCynomolgus TSLP, His Tag (TSP-C52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # A0A2K5TXV0-1).Predicted N-terminus: Tyr 29Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.This protein contains a furin convertase cleavage site, 127-RRKR-130, and will be processed into N and C-terminal fragment with calculated MW of 11.7 kDa and 5.4 kDa respectively. The protein migrates as 21 kDa, 15 kDa and 8 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Cynomolgus TSLP, His Tag (Cat. No. TSP-C52H8) at 5 μg/mL (100 μL/well) can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with a linear range of 2-31 ng/mL (QC tested).
Immobilized Cynomolgus TSLP, His Tag (Cat. No. TSP-C52H8) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 1-16 ng/mL (Routinely tested).
- BackgroundThymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
- References
- (1)Segawa R., et al., 2017, J. Dermatol. Sci. S0923-1811:31012-31015.
- (2)Wang J., et al., 2008, Cell. Mol. Immunol. 5:99-106.
- (3)Soumelis V., et al., 2002, Nat. Immunol. 3:673-680.
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