- SynonymCD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90
- SourceBiotinylated Human Transferrin R, His,Avitag (TFR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # P02786-1).Predicted N-terminus: GlyRequest for sequence
- Molecular Characterization
This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 78.7 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human Transferrin R, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human Transferrin R, His,Avitag(Cat. No. TFR-H82E5) was more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.
Immobilized Five-Minute 6-plex Mouse mAb Isotyping Kit (Cat. No. E001-K001) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, His,Avitag (Cat. No. TFR-H82E5) with a linear range of 2-63 ng/mL (QC tested).
- BackgroundCD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
