- SynonymTNFRSF10D,CD264,TRAILR4,DCR2,TRUNDD
- SourceHuman TRAIL R4, His Tag (TR4-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 56 - His 211 (Accession # NP_003831).Predicted N-terminus: Ala 56Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.7 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human TRAIL R4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundTumor necrosis factor receptor superfamily member 10D (TNFRSF10D) is also known as Decoy receptor 2 (DcR2), TNF-related apoptosis-inducing ligand receptor 4 (TRAIL receptor 4 or TRAIL-R4), CD264,which is the single-pass type I membrane protein. TNFRSF10D is receptor for the cytotoxic ligand TRAIL, which contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis.
- References
- (1)Degli-Esposti M.A., et al., 1997, Immunity 7:813-820.
- (2)Marsters S.A., et al, 1997, Curr. Biol. 7:1003-1006.
- (3)Pan G., et al., 1998, FEBS Lett. 424:41-45.
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