- SynonymSPOCK,TIC1,TICN1,Testican-1
- SourceHuman Testican 1, His Tag (SP1-H5248) is expressed from human 293 cells (HEK293). It contains AA Arg 22 - Trp 439 (Accession # AAH30691).Predicted N-terminus: HisRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 47.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>85% as determined by reduced SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Testican 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
- Citations
Testican-1, as a novel diagnosis of sepsis
Authors: Lee Y, et al.
Journal: J Cell Biochem 2018
Application: Competitive ELISA
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- BackgroundTestican-1 is also known as protein SPOCK，SPOCK1, which contains one azal-like domain and one thyroglobulin type-1 domain.SPOCK1 is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Little is known about SPOCK1"s function until now. It may play a role in cell-cell and cell-matrix interactions except for contributing to various neuronal mechanisms in the central nervous system.
- References
- (1)Alliel P.M., et al., 1993, Eur. J. Biochem. 214:347-350.
- (2)Bonnet F., et al., 1992, Biochem. J. 288:565-569.
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