- SynonymDDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU
- SourceHuman Tau-441, His Tag (TAU-H51H3) is expressed from E.coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 47.9 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by reduced SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, pH7.5. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Tau-441, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundTau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer"s disease, frontotemporal dementia, and other tauopathies. Tau-441 is known as "2N4R," "Isoform Tau-F," "Tau-4" or "Tau 441", which consisting of 441 amino acid. Tau-441 is a potential therapeutic target for pathogenesis.
- References
- (1)Yoshida H, et al. 2012. J. Neurochem. 120:165-176.
- (2)Alonso A., et al. 2001.Proc. Natl. Acad. Sci. U.S.A.98 (12): 6923–8
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