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ACROBiosystems/Human Stathmin 1 / STMN1 Protein, His Tag/100ug/ST1
- SynonymSTMN1,Stathmin,C1orf215,LAP18,OP18
- SourceHuman Stathmin 1, His Tag (ST1-H5149) is expressed from E.coli cells. It contains AA Ala 2 - Asp 149 (Accession # NP_981946).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 18.1 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>92% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Stathmin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
- BackgroundStathmin (STMN1), a member of the stathmin family, is also known as Leukemia-associated phosphoprotein p18, Oncoprotein 18 (Op18), Phosphoprotein p19 (pp19), Protein Pr22 and pp17, which contains one SLD (stathmin-like) domain. The function of STMN1 as an important regulatory protein of microtubule dynamics has been well-characterized. Stathmin (STMN1) interacts with two molecules of dimeric α,β-tubulin to form a tight ternary complex called the T2S complex.One mole of STMN1 binds to two moles of tubulin dimers through the stathmin-like domain (SLD).When STMN1 sequesters tubulin into the T2S complex, tubulin becomes non-polymerizable. Without tubulin polymerization, there is no microtubule assembly.STMN1 also promotes microtubule disassembly by acting directly on the microtubule ends.
- References
- (1)Jourdain L., et al., 1997, Biochemistry. 36(36):10817-21
- (2)Rubin CI., et al., 2004, J Cell Biochem. 93(2):242-50.
- (3)Redeker V., et al., 2000, J. Biol. Chem. 275:6841-6849.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
