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当前位置: 首页 > 产品中心 > cytokine > 顶生系统/生物素化人SOST/硬化蛋白,His标签,超敏(伯胺标记)/25ug/SOT
商品详细顶生系统/生物素化人SOST/硬化蛋白,His标签,超敏(伯胺标记)/25ug/SOT
顶生系统/生物素化人SOST/硬化蛋白,His标签,超敏(伯胺标记)/25ug/SOT
顶生系统/生物素化人SOST/硬化蛋白,His标签,超敏(伯胺标记)/25ug/SOT
商品编号: SOT
品牌: ACROBiosystems
市场价: ¥25000.00
美元价: 12500.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞因子
公司分类: cytokine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  • Synonym
    SOST,VBCH
  • Source
    MABSol® Biotinylated Human SOST, His Tag, primary amine labeling (SOT-H8245) is expressed from human HEK293 cells. It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1). It is the biotinylated form of Human SOST, His Tag (HST-H5245).
    Predicted N-terminus: His
    Request for sequence
  • Molecular Characterization
    Online(Gln 24 - Tyr 213) AAI01087.1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 22.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 4-6 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human SOST, His Tag, primary amine labeling (Cat. No. SOT-H8245) SDS-PAGE gel

Biotinylated Human SOST, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Sclerostin (SOST) also known as Sclerosteosis, VBCH, is a secreted glycoprotein with a signal peptide for secretion and a C-terminal cysteine knot-like (CTCK) domain and belongs to the Cerberus/DAN family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. More recently Sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signalling pathway. Wnt pathway inhibition under these circumstances is antagonistic to bone formation (meaning Sclerostin antagonizes bone formation). It has been shown that SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system. Mutations of Sclerostin is associated with the syndrome Sclerosteosis, and reduced sclerostin expression results in a milder form of the disorder called van Buchem disease.
  • References
    • (1)Li X, et al., 2005, J. Biol. Chem. 280 (20): 19883–7.
    • (2)Van Bezooijen RL, et al., 2007, J. Bone Miner. Res. 22 (1): 19–28.
    • (3)Krause C, et al., 2010, J. Biol. Chem. 285 (53): 41614–26.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍
ACROBiosystems 是一个具有全球范围影响力的生物技术领导品牌,其运营包括了位于北美、 欧洲和亚洲的数家公司, 主要为生命科学研究、生物制药与疫病诊断等行业提供产品、技术服务和临床应用等解决方案,同时致力于在蛋白组学、药物发现和细胞治疗等领域推广面向工业化和临床转化的技术和产品。ACROBiosystems 的业务遍及全球50多个国家和地区,并通过在北美、亚洲、欧洲等世界范围的不同时区设立分支机构以服务于当地客户与经销商。