- SynonymSOD2,IPOB,MNSOD
- SourceHuman SOD2, His Tag (SO2-H5126) is expressed from E.coli cells. It contains AA Lys 25 - Lys 222 (Accession # AAH12423).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.2 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human SOD2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundSuperoxide dismutase [Mn], mitochondrial (SOD2),a member of the iron/manganese superoxide dismutase family, can form a homotetramer and bind one manganese ion per subunit. Theexpression of SOD2 is regulated by KRIT1. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), sporadic motor neuron disease, and cancer. Furthermore, Mn-SOD (SOD2) activity is essential to achieve optimal training-induced protection against both ischemia/reperfusion(IR)-induced cardiac arrhythmias and infarction.
- References
- (1)Choudhary C., et al., 2009, Science 325:83-20°C to -7040.
- (2)Borgstahl G.E.O., et al., 1996, Biochemistry 35:4287-4297.
- (3)Hearn A.S., et al., 2001, Biochemistry 40:12051-12058.
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