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ACROBiosystems/Human SIRP gamma / CD172g Protein, Fc Tag/100ug/SIG
- SynonymSIRPG,CD172g,SIRPB2,SIRP-gamma,SIRP-b2,SIRP-beta-2,Cripto-1
- SourceHuman SIRP gamma, Fc Tag (SIG-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532).Predicted N-terminus: Glu 29Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 63.7 kDa. The protein migrates as 68-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human SIRP gamma, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Human SIRP gamma, Fc Tag (Cat. No. SIG-H5253) with a linear range of 0.01-0.156 μg/mL (QC tested).
- BackgroundSignal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type Imembrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.
- References
- (1)Ichigotani Y., et al., 2000, J. Hum. Genet. 45:378-382.
- (2)Piccio L., et al., 2005, Blood 105:2421-2427.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
