- SynonymSERPINA3,ACT,AACT,GIG24,GIG25
- SourceHuman Serpin A3, His Tag (SE3-H5221) is expressed from human 293 cells (HEK293). It contains AA His 24 - Ala 423 (Accession # AAH03559).Predicted N-terminus: His 24Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.6 kDa. The protein migrates as 50-63 kDaunder reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Serpin A3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundSerpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
- References
- (1)Rubin H., et al., 1990, J. Biol. Chem. 265:1199-1207.
- (2)Kroczynska B., et al., 2004, J. Biol. Chem. 279:11432-11443.
- (3)Liu T., et al., 2005, J. Proteome Res. 4:2070-2080.
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