- SynonymS100A8,60B8AG,CAGA,CFAG,CGLA,CP-10,MRP8,NIF,P8
- SourceHuman S100A8, Tag Free (S18-H5112) is expressed from E.coli cells. It contains AA Met 1 - Glu 93 (Accession # AAH05928).Predicted N-terminus: Met 1Request for sequence
- Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 10.8 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human S100A8, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundS100 calcium binding protein A8 (S100-A8) is also kown as Cystic fibrosis antigen, Migration inhibitory factor-related protein 8 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF,MRP8, NIF and P8, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100A8 plays a role in various functions of myeloid cells by forming a heterocomplex with S100A9. S100A8 and S100A9 are known to be overexpressed in certain species of carcinomas. S100A8 may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. S100A8 plays an important role in differentiation of thyroid carcinoma possibly by forming a complex with S100A9. S100A8 and S100A9 may also play a key role in inflammation-associated cancer.
- References
- (1)Schäfer BW, et al., 1996, Trends Biochem. Sci. 21 (4): 134–40.
- (2)YASUHIRO ITO, et al., 2009, Anticancer Research October, 29 (10 ):4157-4161.
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