- SynonymS100A13,protein S100-A13
- SourceHuman S100A13, Tag Free (S13-H5113) is expressed from E.coli cells. It contains AA Met1 -Lys 98 (Accession # AAH00632).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 11.5 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human S100A13, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundS100 calcium-binding protein A13 (S100A13) is a member of the S-100 family. S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100A13 plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. S100A13 is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine.
- References
- (1)Wicki R., et al., 1996, Biochem. Biophys. Res. Commun. 227:594-599.
- (2)Mandinova A., et al., 2003, J. Cell Sci. 116:2687-2696.
- (3)Cao R., et al., 2010, J. Formos. Med. Assoc. 109:632-640.
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