- SynonymRSPO1,CRISTIN3,FLJ40906,RP11-566C13.1,R-spondin-1
- SourceHuman R-Spondin 1, His Tag (RS1-H4221) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 263 (Accession # Q2MKA7-1).Predicted N-terminus: Ser 21Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.7 kDa. The protein migrates as 38-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human SCARB2, Fc Tag (Cat. No. SC2-H5250) with a linear range of 0.02-0.156 μg/mL (QC tested).
- Citations
NAD+ supplementation rejuvenates aged gut adult stem cells
Authors: Igarashi M, et al
Journal: Aging Cell 2019
Application: Cell Culture
Request for Full-text
AhR activation by 6-formylindolo [3, 2-b] carbazole and 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin inhibit the development of mouse intestinal epithelial cells
Authors: Park JH, et al.
Journal: Environ Toxicol Pharmacol 2016
Application: Cell Culture
Request for Full-text
Glucocorticoids alleviate intestinal ER stress by enhancing protein folding and degradation of misfolded proteins
Authors: Das I, et al.
Journal: J Exp Med 2013
Application: Cell Culture
Request for Full-text
- BackgroundR-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
- References
(1)Chen, JZ. et al. ,2002, Mol Biol Rep. 2002;29:287.
(2)Kim, K.A. et al., 2006, Cell Cycle. 2006;5:23–26.
(3)Nam, J.S. et al., 2007, Gene Expr. Patterns 7, 306–312.
(4)Kazanskaya, O. et al., 2004, Dev. Cell 7:525.
(5)Kamata, T. et al., 2004, Biochim. Biophys. Acta 1676 (1): 51–62.
(6)Binnerts, M.E. et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104 (37): 14700–5.
(7)Wei, Q. et al., 2007, J Biol Chem 282:15903–15911.
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