- SynonymROBO4,ECSM4,MRB
- SourceHuman ROBO4, His Tag (RO4-H5224) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 469 (Accession # AAI11563).Predicted N-terminus: Gln 28Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.9 kDa. The protein migrates as 55-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human ROBO4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundRoundabout homolog 4 (ROBO4) is also known as Magic roundabout (MRB) and ECSM4, which belongs to the immunoglobulin superfamily or ROBO family. ROBO4 contains 2 fibronectin type-III domains and 2 Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is specifically expressed in endothelial cells and is also expressed at sites of angiogenesis in different tumor types. ROBO4 / MRB is the receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO-4 may mediate the inhibition of primary endothelial cell migration by Slit proteins.
- References
- (1)Huminiecki L., et al., 2002, Genomics 79:547-552.
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