- SynonymREG4,GISP,REG-IV,RELP
- SourceHuman Reg4, His Tag (RE4-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 23- Pro 158 (Accession # AAH17089).Predicted N-terminus: Asp 23Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.7 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Reg4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundRegenerating islet-derived protein 4 (REG4) is also known as Gastrointestinal secretory protein (GISP), REG-like protein (RELP). REG4 contains one C-type lectin domain. REG4 has calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. REG4 may be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
- References
- (1)Kaemaeraeinen M., et al., 2003, Am. J. Pathol. 163:11-20.
- (2)Ho M.R., et al., 2010, J. Mol. Biol. 402:682-695.
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