- SynonymAGER,RAGE
- SourceHuman RAGE, Fc Tag (AGR-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ala 344 (Accession # NP_001127).Predicted N-terminus: Ala 23Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>60% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human RAGE, Fc Tag on SDS-PAGEunder reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 60%.
Immobilized Human HMGB1, His Tag (Cat. No. HM1-H5220) at 5 μg/mL (100 μL/well) can bind Human RAGE, Fc Tag (Cat. No. AGR-H5253) with a linear range of 0.078-1.25 μg/mL (QC tested).
- Citations
Nicousamide protects kidney podocyte by inhibiting the TGFβ receptor II phosphorylation and AGE-RAGE signaling
Authors: Sen Zhang, et al.
Journal: Am J Transl Res 2017
Application: SPR
Request for Full-text
- BackgroundAdvanced glycosylation end product-specific receptor (AGER) is also known as RAGE, and is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. AGER is highly expressed in endothelial cells. AGER mediates interactions of advanced glycosylation end products (AGE), and also accumulates in vascular tissue in aging and at an accelerated rate in diabetes, as well as acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGER / RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes.
- References
- (1)Fang F., et al., 2010, FASEB J. 24:1043-1055
- (2)Jin Q., et al., 2011, PLoS ONE 6:E19375-E19375
- (3)Xue J., et al., 2011, Structure 19:722-732
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