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ACROBiosystems/Human PTH1R / PTHR1 Protein, His Tag/100ug/PTR
- SynonymPTH1R,PTHR,PTHR1
- SourceHuman PTH1R, His Tag (PTR-H5222) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Gly 188 (Accession # Q03431-1).Predicted N-terminus: Asp 27Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.6 kDa. The protein migrates as 27-43 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>85% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PTH1R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
- BackgroundParathyroid hormone/parathyroid hormone-related peptide receptor is also known as Parathyroid hormone 1 receptor (PTH1R), PTHR1, PTH/PTHrP type I receptor (PTH/PTHr receptor), which belongs to the G-protein coupled receptor 2 family. PTH1R / PTHR1 is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
- References
- (1)Johnston C.A., et al., 2008, Structure 16:1086-1094.
- (2)Pioszak A.A., et al., 2010, J. Biol. Chem. 285:12435-12444.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
