- SynonymPLA2G1B,Phospholipase A2,PLA2,PLA2A,PPLA2
- SourceHuman PLA2G1B, His Tag (PLB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ser 148 (Accession # AAI06726).Predicted N-terminus: Ala 23Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.0 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Hepes, 150 mM NaCl, pH7.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PLA2G1B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundPhosphatidylcholine 2-acylhydrolase 1B (PLA2G1B) is also known as Phospholipase A2 (PLA2), PLA2A, PPLA2, which belongs to the phospholipase A2 family. PLA2G1B is activated by trypsin cleavage in the duodenum and can also be activated by thrombin or autocatalytically. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
- References
- (1)Grataroli R., et al., 1982, Eur. J. Biochem. 122:111-117.
- (2)Xu W., et al., 2009, J. Biol. Chem. 284:16659-16666.
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