- SynonymPDGFRA,CD140A,PDGFR2,RHEPDGFRA,MGC74795
- SourceHuman PDGF R alpha, His Tag (PDA-H52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 528 (Accession # P16234-1).Predicted N-terminus: Gln 24Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.4 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PDGF R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 2 μg/mL (100 μL/well) can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with a linear range of 0.02-0.625 μg/mL (QC tested).
- BackgroundPDGFRA(platelet-derived growth factor receptor A), also termed PDGFRα(platelet-derived growth factor receptor α), is a receptor located on the surface of a wide range of cell types. This receptor binds to certain isoforms of platelet-derived growth factors (PDGFs) and thereby becomes active in stimulating cell signaling pathways that elicit responses such as cellular growth and differentiation. The receptor is critical for the development of certain tissues and organs during embryogenesis and for the maintenance of these tissues and organs, particularly hematologic tissues, throughout life. Mutations in the gene which codes for PDGFRA, are associated with an array of clinically significant neoplasms.
- References
- (1)Matsui T, et al. 1989. Proc Natl Acad Sci U S A. 86(21):831-20°C to -70.
- (2)Kelly JD, et al. 1991. J Biol Chem. 266(14):8987-92.
- (3)Koschmann C, et al. 2016. Oncotarget. 7(40):65696-65706.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
