- SynonymNCR3,CD337,NKp30,1C7,LY117,MALS
- SourceHuman NKp30, Fc Tag (NC3-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Gly 135 (Accession # AAH52582).Predicted N-terminus: Leu 19Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 39.5 kDa. The protein migrates as 50-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human NKp30, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human B7-H6, His Tag (Cat. No. B76-H52H8) at 10 μg/mL (100 μL/well) can bind Human NKp30, Fc Tag (Cat. No. NC3-H5259) with a linear range of 2-78 ng/mL (QC tested).
- BackgroundNatural cytotoxicity triggering receptor 3 (NCR3) is also known as Activating natural killer receptor p30, Natural killer cell p30-related protein (NK-p30), CD antigen CD337, 1C7, LY117. NCR3 /CD337 /NKp30 belongs to the natural cytotoxicity receptor (NCR) family. NCR3 /NKp30 contains one Ig-like (immunoglobulin-like) domain. NCR3 /NKp30 is selectively expressed by all resting and activated NK cells and weakly expressed in spleen. NCR3 is homodimer in the unliganted form. NCR3 interacts with CD3Z. NCR3 interacts with and is activated by binding to NCR3LG1 or BAG6. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.
- References
- (1)Pende D., et al., 1999, J. Exp. Med. 190:1505-1516.
- (2)Vitale M., et al., 2005, Blood 106:566-571.
- (3)Simhadri V.R., et al., 2008, PLoS ONE 3:E3377-E3377.
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