- SynonymNRP1,Neuropilin-1,NRP,VEGF165R,CD304
- SourceHuman Neuropilin-1, Mouse IgG2a Fc Tag (NR1-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # AAH07533.1).Predicted N-terminus: Phe 22Request for sequence
- Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 96.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 0.1 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Neuropilin-1, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Neuropilin-1, Mouse IgG2a Fc Tag(Cat. No. NR1-H5253) was more than 90% and the molecular weight of this protein is around 230-250 kDa verified by SEC-MALS.
Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 2 μg/mL (100 μL/well) can bind Human Neuropilin-1, Mouse IgG2a Fc Tag (Cat. No. NR1-H5253) with a linear range of 0.2-5 ng/mL (QC tested).
- BackgroundNeuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
