- SynonymMSLN,Mesothelin,MPF
- SourceCynomolgus Mesothelin (296-580), His Tag (MSN-C52H6) is expressed from human 293 cells (HEK293). It contains AA Asp 296 - Gly 580 (Accession # A0A2K5TW94-1).Predicted N-terminus: Asp 296Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.7 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) was more than 90% and around 35-45 kDa verified by SEC-MALS.
Immobilized Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 2-39 ng/mL (Routinely tested).
- BackgroundMesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
- References
- (1)Chang K., et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93:136-140.
- (2)Yamaguchi N., et al., 1994, J. Biol. Chem. 269:805-808.
- (3)Rump A., et al., 2004, J. Biol. Chem. 279:9190-9198.
- (4)Ma J., et al., 2012, J. Biol. Chem. 287:33123-33131.
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