- SynonymM-CSF,CSF-1,Lanimostim
- SourceBiotinylated Human M-CSF, His,Avitag (MCF-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).Predicted N-terminus: Glu 33Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 28.8 kDa. The protein migrates as 35-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human M-CSF, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human M-CSF R, Fc Tag (Cat. No. CSR-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) at2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (Routinely tested).
Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) on SA Biosensor, can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 24.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- BackgroundThe colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
- References
- (1)Sapi E., 2004, Exp. Biol. Med., 229 (1): 1-11.
- (2)Jang Mei-Huei, 2006, The Journal of Immunology, 177 (6): 4055-4063.
- (3)Cao, Qi, 2014, Kidney International, 85 (4): 794-806.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
