- SynonymMBL,MBL2,MBP1,MBP-C,Collectin-1,COLEC1,HSMBPC,MBL2D,MBPD
- SourceHuman MBL, His Tag (MBL-H5220) is expressed from human 293 cells (HEK293). It contains AA Glu 21- Ile 248 (Accession # P11226-1).Predicted N-terminus: Glu 21Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.9 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human MBL, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses
Authors: F Wang, et al
Journal: Mediat Iiflamm 43474
Application: Cell Culture
Request for Full-text
- BackgroundMannose-binding lectin (MBL) is also known as mannose-binding protein, mannan-binding protein (MBP), Mannose-binding protein C, Collectin-1 (COLEC1), MBL2, which belongs to the class of collectins in the C-type lectin superfamily. MBL contains one C-type lectin domain and one collagen-like domain. MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each. MBL is calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells. MBL may bind DNA.
- References
- (1)Nauta A.J., et al., 2003, Eur. J. Immunol. 33:2853-2863.
- (2)Hirano M., et al., 2005, J. Immunol. 175:3177-3185.
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