- SynonymLTBR,D12S370,TNFCR,TNFR3,TNFRSF3,TNFRIII
- SourceHuman LTBR, Fc Tag (LTR-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Met 227 (Accession # P36941-1).Predicted N-terminus: Gln 31Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human LTBR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LTBR, Fc Tag (Cat. No. LTR-H5251) at 5 μg/mL (100 μL/well) can bind Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) with a linear range of 0.3-5 ng/mL (QC tested).
- BackgroundLymphotoxin-beta receptor (LTBR) is also known as Tumor necrosis factor receptor superfamily member 3 (TNFRSF3), Tumor necrosis factor receptor type III (TNF-RIII), which is a single-pass type I membrane protein containing four TNFR-Cys repeat regions. Except for interacting with HCV core protein, LTBR can not only associate with itself, but also can associate with TRAF3, TRAF4 and TRAF5. As the receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT, LTBR promotes apoptosis via TRAF3 and TRAF5. Furthermore, LTBR may play a role in the development of lymphoid organs.
- References
- (1)Rooney I.A., et al., 2000, J. Biol. Chem. 275:14307-14315.
- (2)Nakano H., et al., 1996, J. Biol. Chem. 271:14661-14664.
- (3)Krajewska M., et al., 1998, Am. J. Pathol. 152:1549-1561.
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