- SynonymCLEC4G,LSECtin
- SourceHuman LSECtin, Fc Tag (CLG-H5250) is expressed from human 293 cells (HEK293). It contains AA Lys 55 - Cys 293 (Accession # Q6UXB4-1).Predicted N-terminus: GluRequest for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 52.1 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>85% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human LSECtin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
- Citations
Characterization of a novel anti-human lymphocyte activation gene 3 (LAG-3) antibody for cancer immunotherapy
Authors: X Yu, et al
Journal: MAbs 43642
Application: ELISA
Request for Full-text
Antibodies binding lag-3 and uses thereof
Authors: X Kang, et al
Journal: US20190016800A1 43482
Application: ELISA
Request for Full-text
- BackgroundC-type lectin superfamily 4, member G (CLEC4G) is also known as liver and lymph node sinusoidal endothelial cell C-type lectin (LSECtin), which is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily. CLEC4G / LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner. In addition, CLEC4G / LSECtin has the ability to bind to surface glycoproteins of enveloped viruses. In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin-mediated infection of cells by Ebola virus has been demonstrated.
- References
- (1)Liu W., et al., 2004, J Biol Chem. 279(18):18748-58.
- (2)Powlesland AS., et al., 2008, J Biol Chem. 283(1):593-602.
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