- SynonymLipopolysaccharide-binding protein,LBP
- SourceHuman Lipopolysaccharide, His Tag (LBP-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Val 481 (Accession # P18428-1 (I465F)).Predicted N-terminus: Ala 26Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 52.8 kDa. The protein migrates as 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Lipopolysaccharide, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundLipopolysaccharide-binding protein (LBP),a member of the BPI/LBP/Plunc superfamily, BPI/LBP family, is detected in blood serum. LBP plays a role in the innate immune response. Alao,LBP can bind to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and act as an affinity enhancer for CD14, facilitating its association with LPS. Furthermore, LBP is able to promote the release of cytokines in response to bacterial lipopolysaccharide.
- References
- (1)Schumann R.R., et al., 1990, Science 249:1429-1431.
- (2)Hubacek J.A., et al., 1997, Biochem. Biophys. Res. Commun. 236:427-430.
- (3)Eckert J.K., et al., 2013, Immunity 39:647-660.
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