- SynonymLILRA3,ILT6,LIR4,CD85e
- SourceBiotinylated Human LILRA3, His,Avitag (LI3-H82E0) is expressed from human 293 cells (HEK293). It contains AA Gly 24- Glu439 (Accession # AAH28208.1).Predicted N-terminus: Gly 24Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 48.7 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by reduced SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LILRA3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundLeukocyte immunoglobulin-like receptor subfamily A member 3 (LILRA3) is also known as CD85 antigen-like family member E (CD85e), immunoglobulin-like transcript 6 (ILT-6), and leukocyte immunoglobulin-like receptor 4 (LIR-4) is a protein that in humans is encoded by the LILRA3 gene located within the eukocyte receptor complex on chromosome 19q13.4. Unlike many of its family, LILRA3 lacks a transmembrane domain, which contains 4 Ig-like C2-type (immunoglobulin-like) domains. LILRA3 acts as soluble receptor for class I MHC antigens. At the same time,LILRA3 can bind both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Also,LILRA3 can bind with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.
- References
- (1)Arm J.P., et al., 1997, J. Immunol. 159:2342-2349.
- (2)Borges L., et al., 1997, J. Immunol. 159:5192-5196.
- (3)Ryu M., et al., 2011, PLoS ONE 6:E19245-E19245.
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