- SynonymLILRA1,LIR6,CD85i,LIR-6
- SourceBiotinylated Human LILRA1, His,Avitag (LI1-H82E8) is expressed from human 293 cells (HEK293). It contains AA Pro 17 - Asn 461 (Accession # O75019-1).Predicted N-terminus: Pro 17Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 52.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LILRA1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA1, His,Avitag (Cat. No. LI1-H82E8) with a linear range of 0.313-2.5 μg/mL (QC tested).
- BackgroundLeukocyte immunoglobulin-like receptors (LILRs) are inhibitory, stimulatory or soluble receptors encoded within the leukocyte receptor complex. Some LILRs are extensively polymorphic, and exhibit evidence for balancing selection and association with disease susceptibility. LILRA1 (LIR-6) can recognize MHC (major histocompatibility complex) class I or class I-like molecules, and high levels of sequence similarity among LILRA1, LILRA2 (ILT1), LILRA3 (ILT6) and LILRB1/B2.
- References
- (1)Chen Y., et al., 2009, J. Mol. Biol. 386:841-853.
- (2)Mamegano K., et al., 2008, Genes. Immun. 9:214-223.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
