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Acrobosystems/Human LIGHT/TNFSF14蛋白质，Fc标签（活性三聚体）（MALS验证）/100ug/LIT
- SynonymCD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
- SourceHuman LIGHT, Fc Tag (active trimer) (MALS verified) (LIT-H5269) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1) trimer Design.Predicted N-terminus: ProRequest for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 82.6 kDa. The protein migrates as 116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human LIGHT, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) was more than 90% in HP-SEC, and around 180-200 kDa verified by SEC-MALS.
Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) with a linear range of 0.2-4 ng/mL (QC tested).
Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.29-2.38 ng/mL (Routinely tested).
- BackgroundTumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
- References
- (1)Mauri DN., et al. 1998, Immunity., 8(1), 21-30.
- (2)Granger SW., et al. 2003, Cytokine Growth Factor Rev., 14(3-4), 289-96.
- (3)Pasero C., et al. 2009, Curr Mol Med., 9(7), 911-27.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
