- SynonymKNG1,Kininogen-1,HMWK,BDK,KNG
- SourceHuman Kininogen 1 (LMW), His Tag (KN1-H5225) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Ser 427 (Accession # P01042-2).Predicted N-terminus: Gln 19Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.6 kDa. The protein migrates as 50-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>92% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Kininogen 1 (LMW), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
- BackgroundKininogen-1 (KNG1) is also known as HMWK, BDK, KNG, is a secreted protein which contains three cystatin domains. KNG1 can be cleaved into the following six chains: Kininogen-1 heavy chain, T-kinin, Bradykinin, Lysyl-bradykinin, Kininogen-1 light chain, Low molecular weight growth-promoting factor. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes; LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
- References
- (1)Kellermann J., et al., 1986, Eur. J. Biochem. 154:471-478.
- (2)Wunderer G., et al., 1990, Biol. Chem. Hoppe-Seyler 371:977-981.
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