- SynonymIBP7,IGFBP7
- SourceHuman IGFBP-7, His Tag (IG7-H5240) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # NP_001544).Predicted N-terminus: HisRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 27.0 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IGFBP-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundInsulin-like growth factor-binding protein 7 (IGFBP7) is also known as IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, prostacyclin-stimulating factor and tumor-derived adhesion factor, which contains one Ig-like C2-type (immunoglobulin-like) domain, one IGFBP N-terminal domain and one Kazal-like domain. The major function of IGFBP7 is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity except for IGF-I and IGF-II.IGFBP7 also stimulates cell adhesion. Furthermore, IGFBP7 is implicated in some cancers.
- References
- (1)Yamauchi T., et al., 1994, Biochem. J. 303:591-598.
- (2)Oh Y., et al., 1996, J. Biol. Chem. 271:30322-30325.
- (3)Wilson E.M., et al., 2001, J. Clin. Endocrinol. Metab. 86:4504-4511.
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