- SynonymInterferon-gamma ,Interferon-γ
- SourceActiveMax® Human IFN-gamma, Tag Free (IFG-H4211) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # AAH70256).Predicted N-terminus: Gln 24Request for sequence
- Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 16.8 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to differentglycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human IFN-gamma, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. IF1-H5254) with a linear range of 1-10 ng/mL (QC tested).
The bio-activity of ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL (Routinely tested).
- Citations
Diffusion of cytokines in live lymph node tissue using microfluidic integrated optical imaging
Authors: A.E. Ross, et al.
Journal: ANAL CHIM ACTA 2017
Application:
Request for Full-text
Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells
Authors: Razaghi A, et al.
Journal: Exp Cell Res 2017
Application: Cell Culture
Request for Full-text
- BackgroundInterferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
- References
- (1)Gray PW, et al., 1982, Nature 298 (5877): 859–63.
- (2)Miller CH, et al., 2009, Ann N Y Acad Sci.1182:69-79.
- (3)Saha B, et al., 2010, Cytokine. 50(1):1-14.
- (4)Murphy SP, et al., 2009, Biol Reprod. 80(5):848-59.
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