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ACROBiosystems/Human Iba1 / AIF-1 Protein, His Tag/1mg/IB1
- SynonymIba1,AIF-1,AIF1,G1,IBA1,LCP1
- SourceHuman Iba1, His Tag (IB1-H51H9) is expressed from E.coli cells. It contains AA Met 1 - Pro 147 (Accession # P55008-1).Predicted N-terminus: Met 1Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.6 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Iba1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundAllograft inflammatory factor 1 (AIF1), also known as IBA1, daintain and Protein G1, is an actin-binding protein. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
