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顶生系统/生物素化甲型流感[A/珍珠鸡/香港/WF10/99（H9N2）]血凝素1（HA1）蛋白，His标签，Avitag™/200微克/公顷
![顶生系统/生物素化甲型流感[A/珍珠鸡/香港/WF10/99（H9N2）]血凝素1（HA1）蛋白，His标签，Avitag™/200微克/公顷](https://www.ebiomall.cn/images/ACROBiosystems/HA1-V82E2.jpg)
- SynonymHA1,Hemagglutinin 1
- SourceBiotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA1, His Tag,Avitag (HA1-V82E2) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9-1).Predicted N-terminus: Asp 19Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 39.3 kDa. The protein migrates as 47-70 kDa under reducing (R) condition (SDS-PAGE).
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
![Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA1, His Tag,Avitag (Cat. No. HA1-V82E2) SDS-PAGE gel](images/ACROBiosystems/HA1-V82E2.jpg)
Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA1, His Tag,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundInfluenza, commonly known as "the flu", is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. The serotypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are: H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, H10N7, H7N9. H7N9 is a serotype of the species Influenzavirus A (avian influenza virus or bird flu virus). H7 normally circulates amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in 2013 in China.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
