- SynonymHE4,WAP5,WFDC2,EDDM4
- SourceHuman HE4, His Tag (HE4-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Phe 124 (Accession # AAH46106).Predicted N-terminus: Glu 31Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 10.9 kDa. The protein migrates as 19 kDa and 22-26 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human HE4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
Human epididymis protein 4 antigen‐autoantibody complexes complement cancer antigen 125 for detecting early‐stage ovarian cancer
Authors: Yang WL, et al
Journal: Cancer 2019
Application: Luminex-based immunoassays
Request for Full-text
- BackgroundHuman Epididymis Protein 4 (HE4) is also known as WAP four-disulfide core domain protein 2 (WFDC2), Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, is a member of the WFDC domain family. HE4 / WFDC2 is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is also a small secretory protein, which may be involved in sperm maturation.
- References
(1)Hellström, Ingegerd, et al., 2003, Cancer Research 63 (13): 3695–3700.
(2)Kirchhoff C., et al., 1992, Biol Reprod 45 (2): 350–7.
(3)Schummer M., et al., 1999, Gene 238 (2): 375–85.
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