- SynonymGHR,GHBP,GH receptor
- SourceHuman GHR, His Tag (GHR-H5222) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1).Predicted N-terminus: Ala 27Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human GHR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag (Cat. No. GHR-H5222) with a linear range of 1-8 ng/mL (QC tested).
- Citations
Microfluidic platform for rapid biologics analysis
Authors: Ouyang Wei, et al.
Journal: US20180238915A1 2018
Application: BLI
Request for Full-text
Nanofluidic device for continuous multiparameter quality assurance of biologics
Authors: Ko SH, et al.
Journal: Nat Nanotechnol 2017
Application: Binding Assay
Request for Full-text
The mechanism of immobilized protein A binding to IgG to nanosensor array surfaces
Authors: Justin T. Nelson, et al.
Journal: Anal Chem 2015
Application: SWNT
Request for Full-text
- BackgroundGrowth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
- References
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