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当前位置: 首页 > 产品中心 > Other_natural_proteins > Acrobosystems/人GM-CSF Rα蛋白,His标签(SPR验证)/1mg/GRA
商品详细Acrobosystems/人GM-CSF Rα蛋白,His标签(SPR验证)/1mg/GRA
Acrobosystems/人GM-CSF Rα蛋白,His标签(SPR验证)/1mg/GRA
Acrobosystems/人GM-CSF Rα蛋白,His标签(SPR验证)/1mg/GRA
商品编号: GRA
品牌: ACROBiosystems
市场价: ¥39600.00
美元价: 19800.00
产地: 美国(厂家直采)
公司:
产品分类: 其它天然蛋白
公司分类: Other_natural_proteins
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  • Synonym
    GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,CSF2RA,CSF2R,CSF2RY,CDw116,CD116
  • Source
    Human GM-CSF R alpha, His Tag (SPR verified) (GRA-H52H7) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1).
    Predicted N-terminus: Glu 23
    Request for sequence
  • Molecular Characterization
    Online(Glu 23 - Gly 320) P15509-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human GM-CSF R alpha, His Tag (SPR verified) (Cat. No. GRA-H52H7) SDS-PAGE gel

Human GM-CSF R alpha, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human GM-CSF R alpha, His Tag (SPR verified) (Cat. No. GRA-H52H7) captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, Tag Free (Cat. No. GMF-H4214) with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested).

Protocol
Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human GM-CSF, epitope tag free, primary amine labeling (Cat. No. GMF-H8214) on SA Biosensor, can bind Human GM-CSF R alpha, His Tag (SPR verified) (Cat. No. GRA-H52H7) with an affinity constant of 23.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • Background
    GM-CSF R alpha(Granulocyte-macrophage colony-stimulating factor receptor subunit alpha) is also known as CSF2RA, CD116, GMR-alpha. Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Hereditary pulmonary alveolar proteinosis (hPAP) is a rare disorder of pulmonary surfactant accumulation and hypoxemic respiratory failure caused by mutations in CSF2RA (encoding the granulocyte/macrophage colony-stimulating factor [GM-CSF] receptor α-chain [CD116]), which results in reduced GM-CSF-dependent pulmonary surfactant clearance by alveolar macrophages.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍
ACROBiosystems 是一个具有全球范围影响力的生物技术领导品牌,其运营包括了位于北美、 欧洲和亚洲的数家公司, 主要为生命科学研究、生物制药与疫病诊断等行业提供产品、技术服务和临床应用等解决方案,同时致力于在蛋白组学、药物发现和细胞治疗等领域推广面向工业化和临床转化的技术和产品。ACROBiosystems 的业务遍及全球50多个国家和地区,并通过在北美、亚洲、欧洲等世界范围的不同时区设立分支机构以服务于当地客户与经销商。