- SynonymGlycoprotein G,G,mG,Attachment glycoprotein G,Membrane-bound glycoprotein
- SourceHRSV (A) Glycoprotein G, His Tag (RSG-V5221) is expressed from human 293 cells (HEK293). It contains AA His 67 - Arg 297 (Accession # P20895).Predicted N-terminus: His 67Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.0 kDa. The protein migrates as 46-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
HRSV (A) Glycoprotein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- Citations
IGF1R Is an Entry Receptor for Respiratory Syncytial Virus
Authors: Griffiths, C. D. et al
Journal: Nature 2020
Application: Microscale thermophoresis
Request for Full-text
- BackgroundHuman respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. Human respiratory syncytial virus A (strain Long) major surface glycoprotein G (RSV-G), a member of the pneumoviruses glycoprotein G family, is also known as attachment glycoprotein G and membrane-bound glycoprotein (mG), which contains a linear heparin binding domain essential for virus attachment to the host. Concretely speaking, RSV-G can attache the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Furthermore, RSV-G can also interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, RSV-G lacks both neuraminidase and hemagglutinating activities.
- References
(1)Hendricks D.A., et al., 1986, J. Gen. Virol. 68:1705-1714.
(2)Bao X., et al., 2008, PLoS Pathog. 4 (5): e1000077.
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