- SynonymFZD5,Frizzled-5,C2orf31,,Fz-5,hFz5,FzE5
- SourceHuman Frizzled-5, Fc Tag (FZ5-H5259) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Pro 167 (Accession # Q13467).Predicted N-terminus: Ala 27Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Frizzled-5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
Frizzled-5: a high affinity receptor for secreted frizzled-related protein-2 activation of nuclear factor of activated T-cells c3 signaling to promote angiogenesis
Authors: Peterson YK, et al.
Journal: Angiogenesis 2017
Application: ELISA
Request for Full-text
- BackgroundFrizzled-5 (FZD5) is also known as FzE5, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD5 contains one FZ (frizzled) domain. FZD5 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD5 interacts specifically with Wnt5A to induce the beta-catenin pathway. FZD5 interacts with GOPC.
- References
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