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Acrobosystems/Human Frizzled-10/FZD10蛋白质，His标签（MALS验证）/1mg/FRD
- SynonymFz-10,FZD10,hFz10,FzE7,CD350
- SourceHuman Frizzled-10, His Tag (FRD-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Gly 161 (Accession # Q9ULW2-1).Predicted N-terminus: Ile 21Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.0 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Frizzled-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human Frizzled-10, His Tag (Cat. No. FRD-H52H3) was more than 90% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.
- BackgroundFrizzled-10 (FZD10) is also known as FzE7 or CD350, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD10 contains one FZ (frizzled) domain. FZD10 protein is a cell surface receptor, which is activated by Wnt proteins and involved in the regulation of cellular function. FZD10 interacts with WNT7B and MYOC.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
