- SynonymERBB3,HER3,LCCS2,MDA-BF-1,MGC88033,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3
- SourceHuman ErbB3, His Tag (ER3-H5223) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # P21860-1).Predicted N-terminus: Ser 20Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human ErbB3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ErbB3, His Tag (Cat. No. ER3-H5223) at 2 μg/mL (100 μL/well) can bind Human NRG1 Beta 1, Fc Tag (Cat. No. NR1-H5268) with a linear range of 0.5-8 ng/mL (QC tested).
- Citations
STABILIZED CHIMERIC FABS
Authors: Urosev Dunja, et al.
Journal: US20200308270A1 2020
Application: SPR
Request for Full-text
Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
Authors: J Rohloff et al.
Journal: US9938314B2 2018
Application: SELEX
Request for Full-text
EV20-mediated delivery of cytotoxic auristatin MMAF exhibits potent therapeutic efficacy in cutaneous melanoma
Authors: Capone E, et al.
Journal: J Control Release 2018
Application: ELISA
Request for Full-text
Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities
Authors: Shinozaki N, et al.
Journal: Sci Rep 2017
Application: Rabbit immunization
Request for Full-text
EV20, a novel anti-ErbB-3 humanized antibody, promotes ErbB-3 down-regulation and inhibits tumor growth in vivo
Authors: Sala G, et al.
Journal: Transl Oncol 2013
Application: FACS assay and ELISA
Request for Full-text
- BackgroundErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
- References
(1)Singer, E. etal., 2001, J. Biol. Chem. 276 (47): 44266–74.
(2)Kraus,M.H.etal.,1989,Proc.Natl.Acad.Sci.86:9193-9197.
(3)Carraway,K.L.et al., 1994, J.Biol.Chem., 269: 14303-14306.
(4)Wallasch,C.et al.,1995,EMBO.J.14:4267-4275.
(5)Alimandi,M.et al.,1995,Oncogene.10:1813-1821.
(6)Gamett,D.C.et al.,1995,J.Biol.Chem.270:19022-19027.
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