- SynonymEnvelope protein,Env polyprotein,Envelope glycoprotein,env,COVID-19
- SourceSARS-CoV-2 Envelope protein, GST,His Tag (ENN-C5128) is expressed from E.coli cells. It contains AA Met 1 - Val 75 (Accession # QHU79206.1).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a GST tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.8 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 5 mM EDTA, Arginine, pH8.0. Normally sucrose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
SARS-CoV-2 Envelope protein, GST,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundThe SARS-CoV-2 is composed of a double-layered lipid envelope, including Spike glycoprotein (S), Envelope protein (E), Membrane glycoprotein (M), and Nucleocapsid protein (Nucleocapsid protein, N). Among them, The amino acid sequence of the SARS-CoV-2 envelope protein is 95% identical of the SARS envelope protein.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
