- SynonymCadherin-1,CDH1,CD324,E-cadherin,Arc-1,ECAD,LCAM,UVO
- SourceHuman E-Cadherin, Fc Tag (ECD-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 621 (Accession # AAI41839).Predicted N-terminus: Gln 23Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 92.3 kDa. The protein migrates as 100 kDa and 120 kDa Cadherin, a 48 kDa Propeptide under reducing (R) condition (SDS-PAGE) due to different glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human E-Cadherin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
PAK2 links cell survival to mechanotransduction and metabolism
Authors: Campbell HK, et al
Journal: J Cell Biol 43557
Application: Magnetic bead force assays & 3D atomic force microscopy
Request for Full-text
An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.
Authors: Eshghi A, et al.
Journal: Cell Microbiol 2018
Application: SPR
Request for Full-text
E-cadherin mediated lateral interactions between neighbor cells necessary for collective migration
Authors: Kevin Suffoletto, et al.
Journal: J Biomech 2018
Application:
Request for Full-text
- BackgroundCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).
- References
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