- SourceDinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control was purified from HEK293 cell culture. It is the Biotin labeled form ofMonoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1).
- IsotypeMouse IgG1/kappa
- SpecificitySpecifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
- ApplicationThis antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
5e5 of PBMCs were stained with a series of concentrations of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
- BackgroundA hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
