- SynonymCST2,Cystatin-2,Cystatin-SA,Cystatin-S5
- SourceHuman Cystatin SA, His Tag (CS2-H5224) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Ala 141 (Accession # AAH62679).Predicted N-terminus: Trp 21Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.2 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Cystatin SA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundCystatin-2 (CST2) is also known as Cystatin-SA, Cystatin-S5, is a secreted thiol protease inhibitor, which belongs to the cystatin family. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and is also expressed in submandibular gland and parotid gland.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
