- SynonymCST5,Cystatin-5,Cystatin-D
- SourceHuman Cystatin D, His Tag (CS5-H5220) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Val 142 (Accession # AAH69514).Predicted N-terminus: Gly 21Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.8 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Cystatin D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundCystatin-5 (CST5) is also known as Cystatin-D, is a secreted thermostable protein, which belongs to the cystatin family. CST5 is a cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. CST5 / cystatin-D may play a protective role against proteinases present in the oral cavity.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
